Worms, schistosomiasis, malaria, sleeping sickness trypanosomiasisleishmaniasis 1. They then take in the secondary stain iodine. Compare the API biochemical test results in Figure 2 unknown bacteria from produce with API test results in Figure 3 known bacterial strains identified in the gray boxes on the left.
Example of a particular culture morphology Source Culture morphology When find Identifying bacteria essay unknown bacterium, you first make a pure culture of it on an agar plate. In fact, every species is unique and has particular characteristics.
Genotypic methods Genotypic microbial identification methods can be broken into two broad categories: The parasites may be bacteria,fungi,algae,plants,animals etc…… true parasites usually weaken but rarely kill their hosts. Now that the basics are covered we can move on to start testing on our bacteria to determine which species it might belong to!
Gram-positive bacteria have a thicker peptidoglycan layer and therefore retain the primary stain crystal violet whereas Gram-negative cells lose it when treated with a decolourizer absolute alcohol. Provide results for these two tests Circle or highlight: Get Access Identify the differences between bacteria, viruses, fungi and parasites Essay Sample Bacteria are living things that are neither plants nor animals, but belong to a group all by themselves.
Highlight your responses or use a different font color to distinguish them from the questions themselves. A differential stain is the application of a series of reagents to show species or structural entities.
The chitin adds rigidity and structural support to the thin cells of the fungus, and makes fresh mushrooms crisp Most members of the Identifying bacteria essay Fungi lack flagella; the structures are completely absent in all stages of their life cycle. The bacteria are not rod- or club-shaped, so focus on the cocci arrangements.
An infected cut or ulcer is an example of this. Sample Identification After performing a Gram stain, you observe the following under a microscope: If you use a loop or a needle to transfer bacteria from a medium to another, you must flame the loop or needle in the flame of a Bunsen burner for a few seconds and then wait for the wire to cool down to avoid killing your bacteria.
Changes in climate or introduction of a new species from elsewhere can greatly affect the balance of nature. If you transfer your bacterium to or from a tube, you should flame the neck of the tube for a few seconds before and after.
For example some bacteria are square or star shaped. This is tested on a blood agar plate: Its unique pink rod shaped morphology was the first step observed under the microscope to identify its unknown characteristic. Inhibiting any of these factors can break the chain of bacterial growth.
Eubacteriales, Actinomycetales, Streptomycetales, and Flexibacteriales. This was an indicant of the bacteria interrupting down and metabolising the amylum.
Indeed, we have approximatively human cells and bacterials cells in us.
Deadline range from 6 hours to 30 days. There were other methods utilized in lab as well: The discrimination and identification of bacteria within mixed natural populations is also a rapidly developing field that utilizes some of the same techniques, but it is an entirely separate subject Liu and StahlLogue Identifying bacteria essay al.
Biochemical properties The final step to determining your bacterial species is a series of tests to know its biochemical properties. All those stains give you additional data on your unknown cell and brings you closer to knowing which species it belongs to.
What tests would you perform initially to start you on your way? Azotobacter, as one of the more common nitrifying dirt bugs, is known to bring forth a great sum of useable Nitrogen, and hence is closely linked to works growing and wellness Cakmakci et al.
After performing a gram stain on unknown number five, number five was determined to be gram positive rod. Biochemical properties continued Another test is whether or not your unknown has an hemolytic reaction. They do exhibit enormous biochemical diversity in both their metabolism and cell structure; this has proved to be a useful cue for the taxonomy of some groups, but by no means all of them.
You can either inoculate an agar plate and put it in an anaerobic jar or inoculate your bacteria directly into thioglycolate broth or cooked meat medium. Localised means the infection is restricted to one small area only.
This is not to say that bacterial systematics is now fully standardized; indeed, there is a vigorous ongoing debate about what constitutes a bacterial species Gevers et al.
Stanier and van Neil  recognized the Kingdom Monera with 2 phyla, Myxophyta and Schizomycetae, the latter comprising classes Eubacteriae 3 ordersMyxobacteriae 1 orderand Spirochetae 1 order.
After that, specific biochemical tests were performed for gram positive, since unknown number five was determined to be gram positive rod.There are two main types of bacteria, gram negative and gram-positive.
The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number The following are the tests that were used to identify the two different bacteria.
Bacteria identification is accomplished in a number of ways. Two common tools microbiologists use to identify unknown bacteria include dichotomous key and biochemical tests.
The dichotomous key is useful when a microbiologist only needs to know which group an unknown microbe belongs to on a general level. Although the use of mass spectrometry has great potential for identifying bacteria by their spectral profile, many factors affect the reproducibility of bacterial spectra.
Sample preparation, matrix selection, and differences in instrument quality and performance can all have an impact on the reproducibility of protein profiles. Bacteria are microscopic single-celled organisms that can be helpful, such as those that live in our guts, or harmful, such as flesh-eating bacteria.
In his essay. Identifying an unknown bacterial strain. Updated on December 8, Ophelie Quillier. more. Contact Author. Why identify a bacteria?
Bacteria are everywhere, they are part of our environment and even of us. In fact, we are more bacteria than human!
Identifying bacteria can lead to many new discoveries such as new species or the evolution of a known species. In this experiment, an unknown bacterium was to be identified using a number of different tests using several different bacteria for comparison.Download